Submitted by jec3011 on May 9, 2023 - 12:33pm
| Title | Protocol for Plasma Extracellular Vesicle and Particle Isolation and Mass Spectrometry-Based Proteomic Identification. |
| Publication Type | Journal Article |
| Year of Publication | 2023 |
| Authors | Kenari ANasiri, Bojmar L, Heissel S, Molina H, Lyden D, Hoshino A |
| Journal | Methods Mol Biol |
| Volume | 2628 |
| Pagination | 291-300 |
| Date Published | 2023 |
| ISSN | 1940-6029 |
| Keywords | Blood Proteins, Extracellular Vesicles, Humans, Mass Spectrometry, Proteome, Proteomics, Ultracentrifugation |
| Abstract | Plasma extracellular vesicles and particles (EVPs) are enriched in biomolecules that reflect individuals' physiological and pathological states. Several studies have demonstrated the potential of human plasma EVPs as a novel liquid biopsy. Here we describe a protocol for human plasma EVPs isolation and proteomic characterization. We isolated human plasma EVPs by the classical ultracentrifugation method and performed mass spectrometry-based proteomic profiling. Using this protocol, researchers can reveal the plasma EVPs proteome and explore the clinical application of plasma EVPs proteins for developing disease biomarkers. |
| DOI | 10.1007/978-1-0716-2978-9_19 |
| Alternate Journal | Methods Mol Biol |
| PubMed ID | 36781793 |