Submitted by jec3011 on April 17, 2023 - 4:37pm
| Title | Non-reversible tissue fixation retains extracellular vesicles for in situ imaging. |
| Publication Type | Journal Article |
| Year of Publication | 2019 |
| Authors | Gupta MP, Tandalam S, Ostrager S, Lever AS, Fung AR, Hurley DD, Alegre GB, Espinal JE, H Remmel L, Mukherjee S, Levine BM, Robins RP, Molina H, Dill BD, Kenific CM, Tuschl T, Lyden D, D'Amico DJ, Pena JTG |
| Journal | Nat Methods |
| Volume | 16 |
| Issue | 12 |
| Pagination | 1269-1273 |
| Date Published | 2019 Dec |
| ISSN | 1548-7105 |
| Keywords | Animals, Carbodiimides, Cattle, Cell Line, Tumor, Extracellular Vesicles, HEK293 Cells, Humans, Male, Mice, Mice, Inbred C57BL, Microscopy, Electron, Transmission, Tissue Fixation, Vitreous Body |
| Abstract | Extracellular vesicles (EVs) are secreted nanosized particles with many biological functions and pathological associations. The inability to image EVs in fixed tissues has been a major limitation to understanding their role in healthy and diseased tissue microenvironments. Here, we show that crosslinking mammalian tissues with formaldehyde results in significant EV loss, which can be prevented by additional fixation with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) for visualization of EVs in a range of normal and cancer tissues. |
| DOI | 10.1038/s41592-019-0623-4 |
| Alternate Journal | Nat Methods |
| PubMed ID | 31712780 |
| PubMed Central ID | PMC9344330 |
| Grant List | L30 EY021921 / EY / NEI NIH HHS / United States R35 CA232093 / CA / NCI NIH HHS / United States U19 CA179564 / CA / NCI NIH HHS / United States |